C3a, Human, ELISA kit – 2 x 96 det. - HK354-02
Quantity
2 x 96 det.
Catalog #
HK354-02
1.153,00 €
Verwandte Produkte
C3a, a vital component in innate immunity, central to complement activation, rapidly converts in serum to its stable form, C3a-desArg, offering a reliable measure of immune response. This transformation is key for researchers tracking inflammation and immune reactions in conditions like sepsis, heart disease, and autoimmune disorders. Our precision-focused human C3a ELISA kit, targeting a unique neo-epitope on C3a-desArg, guarantees accurate results without interference from C3. This makes C3a an indispensable biomarker for groundbreaking immunological research.
| Datasheet URL | https://www.hycultbiotech.com/wp-content/uploads/2022/06/HK354-1223.pdf |
|---|---|
| Quantity | 2 x 96 det. |
| Quantity | 2 x 96 det. |
| Standard range | 31.3 to 2000 pg/ml |
| Detection level | 31.3 pg/ml |
| Working volume | 100 µl/well |
| Species | human |
| Cross reactivity | Cynomolgus monkey - Yes, Pig - No |
| Application | The human C3a ELISA kit is to be used for the in vitro quantitative determination of human C3a/C3a-desArg in serum, plasma, bronchoalveolar lavage fluid (BALF) and urine samples. |
| Principle | The human C3a ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C3a. Biotinylated tracer antibody will bind to captured human C3a. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human C3a standards (log). The human C3a concentration of samples, which are run concurrently with the standards, can be determined from the standard curve. |
| Storage and stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months. |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. |
| References | 1. Morad H et al; Time-course analysis of C3a and C5a quantifies the coupling between the upper and terminal Complement pathways in vitro. J Immunol Meth 2015, 427: 13 2. Hartmann H et al; Rapid quantification of C3a and C5a using a combination of chromatographic and immunoassay procedures. J Immunol Meth 1993, 166: 35 3. Stove S at al; Re-evaluation of the storage conditions for blood samples which are used for determination of complement activation. J Immunol Meth 1995, 182: 1 4. Roy, R et al; Complement component 3C3 and C3a Receptor are required in chitin-dependent allergic sensitization to Aspergillus fumigatus but dispensable in chitin-induced innate allergic inflammation. Molecular Biology 2013, 4: 2 5. Sethu, S et al; Immunoglobulin G1 and immunoglobulin G4 antibodies in multiple sclerosis patients treated with IFNβ interact with the endogenous cytokine and activate complement. Clin Immunol 2013, 148: 2 |
| Disease | Infectious diseases, Nephrology |
| Application assays: | The human C3a ELISA kit is to be used for the in vitro quantitative determination of human C3a/C3a-desArg in serum, plasma, bronchoalveolar lavage fluid (BALF) and urine samples. |
|---|---|
| Principle: | The human C3a ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human C3a. Biotinylated tracer antibody will bind to captured human C3a. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human C3a standards (log). The human C3a concentration of samples, which are run concurrently with the standards, can be determined from the standard curve. |
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