VitroGel STEM (10 mL)

- Establish stem cell spheroids directly from liquid nitrogen (5 min protocol)
- High-quality 3D stem cells for differentiation and organoid formation
- Easy scale-up (from laboratory scale to industrial scale)
- Efficient cell harvesting or subculturing

VitroGel® STEM is a xeno-free (animal origin-free) hydrogel system developed to improve the performance of three-dimensional (3D) static suspension cultures and scale-up of human pluripotent stem cells (hPSCs) to create a high-throughput system to model various tissue and disease states.

This hydrogel system is ready to use with an optimized formulation that fully supports the rapid expansion of high-quality 3D stem cell spheroids with pluripotent properties. hPSCs directly thawed from liquid nitrogen or passaged from 2D matrix coated culture vessels can be immediately mixed with the hydrogel solution for static suspension cultures. Moreover, the optimization protocol is ideal for time-sensitive experiments, as it does not require excessive medium exchanges, which can ultimately save on time and materials. This hydrogel system is compatible with most hPSC culture media and tissue culture vessels. Due to the unique static suspension culture procedure, the requirement for microcarriers for large-scale bioreactors is eliminated, making cell harvesting simple and effective. The 3D stem cell spheroids that are developed using this system can be used for further sub-cultures, patterned differentiations, organoid development, or re-establishing 2D culture morphologies.