VitroGel ORGANOID-3 (10 mL)

- Xeno-free hydrogel for organoid formation
- Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources
- Scalable protocol for lab automation
- Easy 20-minute cell harvesting (enzyme-free)

VitroGel® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.

The VitroGel ORGANOID Discovery Kit (Cat# VHM04-K) includes all four types of organoid hydrogels (VitroGel ORGANOID-1, VitroGel ORGANOID-2, VitroGel ORGANOID-3, VitroGel ORGANOID-4) which were formulated with various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions. Use the Discovery Kit to screen which organoid hydrogel version works best for your organoid culture.

VitroGel ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.

The organoid hydrogels can work in conjunction with VitroGel STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel STEM to VitroGel ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow B. Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution. VitroGel ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.