4-HNE(4-Hydroxynonenal) ELISA Kit - ELK9581

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Catalog #
ELK9581
Ab 360,00 €
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Weitere Informationen
Datasheet URL https://www.elkbiotech.com/upload/file/ELISA/ELK9581-1.pdf
Alternative Names 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal
Sensitivity 0.38 ng/mL
Standard range 40 ng/mL
Detection Range 0.63-40 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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